productdatasheet


Glypican-1MonoclonalAntibody


ORDERINGINFORMATION

CatalogNo.:34029

Size:100ugProteinG-purifiedantibodyinPBS,pH7.4.

SPECIFICATIONSUMMARY

Antigen:Recombinantfull-lengthhumanglypican-1.

Cloneno.:9E9

HostSpecies:MouseAntibodyClass:IgG1Stabilizers:None

Preservatives:None.Availableonrequest.

BACKGROUND

Glypican-1isawidelyexpressed,GPIanchored,cellsurfaceproteoglycanthathasbeenreportedtobeimportantincellularadhesionandmigration.

Glypican-1alsofunctionsasanaccessorymoleculeforsignalingreceptors.Glypican-1isoverexpressedinhumangliomasandpancreaticductalcellcarcinomas.Expressionofglypican-1isassociatedwiththeaggressivebehavioroftumorcellsinvitroandinvivo.

SPECIFICITY

Thisantibodyrecognizesnativeandrecombinanthumanandmouseglypican-1.

APPLICATIONS

ELISA:at1-5ug/mlwilldetectrecombinantglypican-1onthesolidphaseat200ng/well.

APPLICATIONS(continued)Immunoblotting:at1-10ug/ml,abandofapprox.64kDaisdetectedinnormalmousebrain.

Immunofluorescence:at1-10ug/ml,positivestainingofhumanpancreaticcarcinomacelllineCapan-1.

Thesearerecommendedconcen-trations.Usershoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone

(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.