product data sheet


Recombinant Human DnaK (Hsp70), His


Catalog Number

Size

11116P-10

10ug

11116P-50

50ug

11116P-1000

1000ug

Catalog Number

Size

11116P-10

10ug

11116P-50

50ug

11116P-1000

1000ug

ORDERING INFORMATION


Formulation: Sterile-filtered colorless solution contains 20mM Tris, pH 7.5, and 2mM DTT having a protein concentration of 1mg/ml. Purified by proprietary chromatographic techniques.


BACKGROUND

Hsp70 plays an important role in protein folding and in protecting cells from stress. In most species there are many proteins that belong to the Hsp70 family. Some are expressed only under stress conditions while others are present in cells under normal conditions and are not heat-inducible.


DESCRIPTION

Recombinant Human DnaK (Hsp70) produced in E. coli is a single, non- glycosylated polypeptide chain containing 661 amino acids fused to His-tag at the N-terminus with a total molecular weight of 72.2kDa.


SPECIFICATION SUMMARY

Source: Escherichia coli

Purity: Greater than 95% as determined by SDS-PAGE and RP- HPLC.


Accession number: P08107

Amino Acid Sequence:

MGSSHHHHHH SSGLVPRGSH MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPSYVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDKPKVQVSYKGD TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKDAGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVKATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLSLGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFELSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQEAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEVD


STORAGE AND STABILITY

Although stable at 4oC for 4 weeks, product should be stored at or below

-20oC. For long term storage, addition

of a carrier protein (0.1% HSA or BSA) is recommended.


For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.


QED Bioscience, Inc. 10919 Technology Place, Suite C

San Diego, CA 92127

Toll Free 800.929.2114

Phone 858.675.2405

Fax 858.592.1509

info@qedbio.com

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