TransmissibleGastroenteritisVirus(TGEV)MonoclonalAntibodies


LibraryPackNo.100ug/clone

180101All4clones

LibraryPackNo.100ug/clone

180101All4clones

ORDERINGINFORMATION

CatalogNo.CloneNo.MAbSubtypeSize

18001TGEV10-116.3.3IgG1100ug,500ug

18002TGEV11-314.6IgG1100ug,500ug

18003TGEV15-886.6IgG2a100ug,500ug

18004TGEV15-948.2.1IgG2a100ug,500ug

Format:PurifiedantibodyinPBS,pH7.4


BACKGROUND

TransmissibleGastroenteritis(TGEV)isanenvelopedCoronaviruswithapositive-sensesingle-strandedRNAgenomeandahelicalsymmetry.Thegenomicsizeofcoronavirusesisapproximately28.6kilobases.ProteinsthatcontributetotheoverallstructureofTGEVincludethespike(S),envelope(E),membrane(M)andnucleocapsid(N).OthercoronavirusesbelongingtothisfamilyincludeFelinecoronavirus(FeCV),Caninecoronavirus(CCV),andFelineinfectiousperitonitisvirus(FIPV).TGEVinfectspigs.However,inyoungpigs,themortalityrateiscloseto100%.ThepathologyofTGEVissimilartothatofothercoronaviruses.Coronavirusesenterthehostbyfirstattachingtothehostcellusingthespikeglycoprotein.TheSproteininteractswiththeporcineaminopeptidaseN(pAPN),acellularreceptor,toaideinitsentry.AdomainintheSspikeproteinisrecognizedbypAPN,andtransfectionofpAPNoccurstononpermissivecellsandinfectsthemwithTGEV.Oncethevirusinfectsthehost,itmultipliesinthecellliningofthesmallintestineresultinginthelossofabsorptivecellsthatinturnleadstoshorteningofvilli.Theinfectedswinethenhasreducedcapabilityfordigestingfoodanddiefromdehydration.


SPECIFICATIONSUMMARY

Antigen:TGEV-infectedSTcells

HostSpecies:Mouse

Specificity:TheseantibodiesrecognizeTGEVanddonotcross-reactwithFIPV,FeCV,orCCV.

#18003and#18004canneutralizeTGEVinvitro.


APPLICATIONS

TheseantibodieshavebeenqualifiedforuseinELISAtodetectTGEV.#18003and#18004maybeusedinTGEVneutralizationassays.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Theseantibodiesarestableforatleastone(1)yearat-20oto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.