Hsp70/Hsc70 (Plant) Monoclonal Antibody


Catalog No.: 11138 (clone 5G1-95) Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.

Purified by Protein G affinity chromatography.


Hsp70 genes encode heat-inducible 70kDa heat shock proteins. Hsp70 binds ATP with high affinity and possesses weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. The structure of the ATP binding domain displays multiple features of nucleotide binding proteins. The binding of ATP triggers a critical conformational change which leads to the release of the bound substrate protein. The universal ability of Hsp70s to undergo cycles of binding to and release from partially unfolded proteins is the basis of their role in protein synthesis, protein folding, oligomerization, and protein transport.


Antigen: Purified Hsp70 from Phaseolus aureus (mung bean). Host Species: Mouse Antibody Class: IgG1


This antibody recognizes constitutive and inducible plant Hsp70. It does not cross-react with human, rat, bacteria (DNAKK) or human BIP.


Immunoblotting: use at 1ug/ml. A band of

~70kDa is detected.

Western blot detection of Hsp70 in Duckweed (L.minor) cells with #11138.

ELISA: use at 1-10ug/ml.

These are recommended concentrations; enduser should determine optimal concentrations for their applications.


Dilute in PBS or medium which is identical to that used in the assay system.


This antibody is stable for at least one (1) year at -20oC. Avoid repeated freeze- thaw cycles.

For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.