productdatasheet


RecombinantHumanVascularEndothelialGrowthFactor


CatalogNumber

Size

45109P-2

2ug

45109P-10

10ug

45109P-1000

1000ug

CatalogNumber

Size

45109P-2

2ug

45109P-10

10ug

45109P-1000

1000ug

ORDERINGINFORMATION


Format:Sterilefiltered,lyophilized.

Purification:Chromatographic


DESCRIPTION

RecombinantHumanVEGFproducedin

E.coliisadouble,non-glycosylated,polypeptidechaincontaining165aminoacidswithamolecularmassof38,231daltons.ThisproducthasfullBIOLOGicalactivity.


SPECIFICATIONSUMMARY

Source:EscherichiacoliStABIlizers:NonePreservatives:None

Purity:Greaterthan98%byRP-HPLC,anion-exchangechromatography;reducingandnon-reducingSDS-PAGE,silverstain.

Dimers/Aggregates:<1%bySDS-PAGE

BiologicalActivity:Thebiologicalactivityisdeterminedbythedose-dependentstimulationofthe

proliferationofhumanumbilicalveinendothelialcellsusingaconcentrationof1.0to8.0ng/ml.


Endotoxin:<0.1ng/ug

ProteinContent:Determinedspectrophotometricallyat280nm(extinctioncoefficient0.2875)andbyRP-HPLCanalysisagainstacalibratedstandard.


SOLUBILITY

Reconstitutethelyophilizedproductinsterilewaternotlessthan100ug/ml.


STORAGEANDSTABILITY

Lyophilizedproductisstableatroomtemperaturefor3weeks.Storelyophilizedproductdesiccatedat-20°C.Uponreconstitution,storeat4°Cfor2-7daysoraliquotandstoreat-20°C.Forlong-termstorageaddeitherHSAorBSAto0.1%.Avoidrepeatedfreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures



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Phone858.675.2405

Fax858.592.1509

info@qedbio.com


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