c-mycAffinity-PurifiedPolyclonalAntibody,HRPconjugate


ORDERINGINFORMATION

CatalogNo.Size

18826P100ug

Format:1mg/mlconjugatedtohorseradishperoxidase(HRP)inPhosphateBufferedSaline,pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.

BACKGROUND

c-mycisanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.ThepeptidesequenceofthemyctagisEQKLISEEDL(Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu).Thec-myctagisapproximately1202Daltonsinatomicmassandincludes10aminoacids.

SPECIFICATIONSUMMARY

Antigen:Aminoacids410-419(EQKLISEEDL)ofhumanmycconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

ELISA:useatadilutionof1:10,000-1:100,000.


Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.


Detectionofc-myc-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18826Pdiluted1:25,000.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.